HAN-P-15
Robert E. Campbell
The University of Tokyo
Professor
Japan
Fluorescent Proteins, Protein Engineering, Biosensors
Academic Career
B.Sc., 1994, University of British Columbia; Ph.D., 2000, University of British Columbia (Advisor: Martin E. Tanner); Postdoctoral training, 2000-2003, University of California, San Diego (Advisor: Roger Y. Tsien); Assistant Professor, 2003-2009, University of Alberta; Associate Professor, 2009-2013, University of Alberta; Professor, 2013-2023, University of Alberta; Professor, 2018-present, The University of Tokyo.
Selected Publications
D. Cheng, Z. Ouyang, X. He, Y. Nasu, Y. Wen, T. Terai*, and R.E. Campbell*, “A high-performance chemigenetic potassium ion indicator”, J. Am. Chem. Soc., 2024, accepted.
S. Takeuchi, S. Imai, T. Terai* and R.E. Campbell*, “A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ion”, RSC Chem. Biol., 2024, accepted.
F. Chai, H. Fujii, G.N.T. Le, C. Lin (equal contribution), K. Ota (equal contribution), K.M. Lin, L.M.T. Pham, P. Zou, M. Drobizhev, Y. Nasu, T. Terai, H. Bito, and R.E. Campbell*, “Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons”, ACS Sens., 2024, 9, 3394–3402.
S. Hario, G.N.T. Le, H. Sugimoto, K. Takahashi-Yamashiro, S. Nishinami, H. Toda, S. Li, J.S. Marvin, S. Kuroda, M. Drobizhev, T. Terai, Y. Nasu*, and R.E. Campbell*, “High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate”, ACS Cent. Sci., 2024, 10, 402–416. Preprint posted to bioRxiv 2022.10.19.512892.
Y. Nasu*, A. Aggarwal, G.N.T. Le, C.T. Vo, Y. Kambe, X. Wang, F.R.M. Beinlich, A.B. Lee, T.R. Ram, F. Wang, K.A. Gorzo, Y. Kamijo, M. Boisvert, S. Nishinami, G. Kawamura, T. Ozawa, H. Toda, G.R. Gordon, S. Ge, H. Hirase, M. Nedergaard, M.-E. Paquet, M. Drobizhev, K. Podgorski, and R.E. Campbell*, “Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging”, Nat. Commun., 2023, 14, 6598. Preprint posted to bioRxiv 2022.12.27.522013.
W. Zhu, S. Takeuchi, S. Imai, T. Terada, T. Ueda, Y. Nasu, T. Terai* and R.E. Campbell*, “Chemigenetic indicators based on synthetic chelators and green fluorescent protein”, Nat. Chem. Biol., 2023, 19, 38-44.
K.K. Tsao*, S. Imai, M. Chang, S. Hario, T. Terai*, R.E. Campbell*, “The best of both worlds: Chemigenetic fluorescent sensors for biological imaging”, Cell Chem. Biol., 2024, 31, 1652-1664.
Y. Nasu, Y. Shen, L. Kramer, and R.E. Campbell*, “Structure- and mechanism-guided design of single fluorescent protein-based biosensors”, Nat. Chem. Biol. 2021, 17, 509–518.
Why My Lab?
Our expertise is in the area of protein engineering, particularly as it relates to the development of fluorescent protein-based biosensors for biological imaging applications. Our research sits squarely at the interface of chemistry and biology and the tools that we produce are used by biologists in hundreds of labs around the world.
